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State of decay 2 surviving 100 days tips11/14/2023 Here, we present next-generation CRISPRi sgRNA libraries and effector expression constructs that enable strong and consistent knockdown across mammalian cell models. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biology, genetics, and biotechnology, but wider deployment of CRISPRi screening has been constrained by the large size of single guide RNA (sgRNA) libraries and challenges in generating cell models with consistent CRISPRi-mediated knockdown. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, United States ĬRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Department of Biology, Massachusetts Institute of Technology, United States.Department of Biochemistry and Biophysics, University of California, San Francisco, United States.Department of Urology and Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, United States.National Institute of Neurological Disorders and Stroke, National Institutes of Health, United States.National Institute on Aging, National Institutes of Health, United States.Center for Alzheimer's Disease and Related Dementias, National Institutes of Health, United States.Institute for Neurodegenerative Disease, University of California, San Francisco, United States.Department of Microbiology, Harvard Medical School, United States.Whitehead Institute for Biomedical Research, United States.Howard Hughes Medical Institute, Massachusetts Institute of Technology, United States. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, United States.Medical Scientist Training Program, University of California, San Francisco, United States.
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